Insertion junction: CLIP2.019602_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019602
Chromosome:chromosome 3
Location:1863565
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g154600 (1 of 2) PTHR35514:SF1 - THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACAGTGTTGTCATTGGCTACGACGCCACGGCGCCCTCGCTCATCAACG
Internal bar code:ATTTATCTGGAGTGTTTAAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:286
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCATGAGCCTACCTAGCC
Suggested primer 2:TACGACACGACACAAGCACA