| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.019613 |
| Chromosome: | chromosome 12 |
| Location: | 4100661 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801402 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGTCCCGCACGGCAACGAGACCTCACCGTCGTACATAGCATTCACAT |
| Internal bar code: | TGTTTTGATGGTGATGACTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3437 |
| LEAP-Seq percent confirming: | 55.3191 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTATGTCGATCAGCGTGA |
| Suggested primer 2: | GCGTCATGCCGAATTTCTCC |