Insertion junction: CLIP2.019618_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019618
Chromosome:chromosome 10
Location:5026014
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g454700 (1 of 1) IPR001611//IPR003591//IPR025875 - Leucine-rich repeat // Leucine-rich repeat, typical subtype // Leucine rich repeat 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACCTGCCCGGCCCACGAGTGCCGGGAGGTCGGTTCCCAAGAAGCTGAC
Internal bar code:GCGTGATCCAAGCACTCCAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2381
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAACCACTGCCCACATTG
Suggested primer 2:AGGAGGACGAGGTAGAGCTG