| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019619 |
| Chromosome: | chromosome 2 |
| Location: | 6981034 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389430 | TSF3 | (1 of 1) PTHR11538:SF40 - PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT; Phenylalanyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTATAACCCTGGTGCAGGGGCCACACAGACGTTTGTGTACATTAAAA |
| Internal bar code: | TCTATTAACTCTTATTCGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3473 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCGAGACTGTGGAATGA |
| Suggested primer 2: | CAAATGGTGGCATATGGCGG |