Insertion junction: CLIP2.019631_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019631
Chromosome:chromosome 16
Location:6844685
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g671850 (1 of 9) PF01490 - Transmembrane amino acid transporter protein (Aa_trans) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGACCATTGCTACAGCCATGGCGCCCGACCCCAGCACCGACGTCCAGC
Internal bar code:AAGGCTAGATAACGACACCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:776
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGATGTGGGTCATGGCTT
Suggested primer 2:AAACAAAGAGACACGCACGC