| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019650 |
| Chromosome: | chromosome 16 |
| Location: | 5067938 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685800 | (1 of 4) 3.1.2.2//3.1.2.20 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase // Acyl-CoA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTGGAGGCGGCTTGTTTGGGTGCGGCTGCGGCTGGTGGTGTTTGTGG |
| Internal bar code: | TCATTGGTGTAGTTCTGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2483 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGAACCCAATGCACGTC |
| Suggested primer 2: | TCACGAGTCACGACCCAAAG |