Insertion junction: CLIP2.019653_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.019653
Chromosome:chromosome 2
Location:5712467
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g116350 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGTAGGTTGGGTCTGACGCTAGCCGTTGTTGCCTTACGGGGGTCAGC
Internal bar code:TCGTTGGTCGTATTTTGTTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTACTTCGTTGGGCTTGG
Suggested primer 2:TAGGCATCCAGGTCATCCCA