Insertion junction: CLIP2.019657_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.019657
Chromosome:chromosome 3
Location:199168
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144164 RSE2,RSEP2 (1 of 1) PTHR22939:SF79 - PEPTIDASE M50 FAMILY PROTEIN; Intramembrane metalloprotease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGACATATACATACCGCCTCGTTGCCACACTGACCGCAACAGTACCCG
Internal bar code:TGATCTCTATTCAAAGTAGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:662
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTCACCGTGTCATACA
Suggested primer 2:CACACACTCTCTCACCCACC