| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.019669 |
| Chromosome: | chromosome 16 |
| Location: | 2601222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661100 | MAPK7 | (1 of 1) K08829 - male germ cell-associated kinase [EC:2.7.11.22] (MAK); Mitogen-Activated Protein Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCTGTGGTGTGCGCGAGCCAGCGTGCACGCTGCCGCCGCCAGGATGCG |
| Internal bar code: | GTCTAGTGTGTGAACTTAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2432 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATGCAGGGGTGGTTCAG |
| Suggested primer 2: | GCCTGCCTTGCTAGAGGAAA |