Insertion junction: CLIP2.019671_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019671
Chromosome:chromosome 1
Location:57649
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g000250 SNR1 (1 of 2) PTHR12677:SF5 - TVP38/TMEM64 FAMILY MEMBRANE PROTEIN YDJX; Predicted SNARE-associated Golgi protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAAAAATGTGAAGAACGAGGTAGAATCTGCCACCGTGGGTCTCCAGCG
Internal bar code:GGGTTAACCGTAAGATTGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2433
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAGGCAAGGTGTGACAG
Suggested primer 2:GAGTGTTGAACCGAGACCGT