| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.019675 |
| Chromosome: | chromosome 12 |
| Location: | 7280973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801466 | (1 of 2) IPR028992 - Hedgehog/Intein (Hint) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGTACGGCACCTGAGGGGAGGGAATGCAGGCTTGTCAGTTAAAAGC |
| Internal bar code: | AAACTTGAGATTATACTGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3405 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCACAGAGGAGAACCCAG |
| Suggested primer 2: | CACGTCATGATCGTCAACGC |