Insertion junction: CLIP2.019686_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019686
Chromosome:chromosome 5
Location:980321
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247050 CTR4,COPT1 CTR type copper ion transporter; (1 of 2) K14686 - solute carrier family 31 (copper transporter), member 1 (SLC31A1, CTR1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACCATGCCGCGCACAGCCACGGCGCTAGCAACACGCCCGCTCCATCCG
Internal bar code:GGAGATGTCTTCAAGTCGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2293
LEAP-Seq percent confirming:96.9697
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCGCCACTCACTTCTGTC
Suggested primer 2:CAAGAAGTTCGCAAGTGCCC