| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019707 |
| Chromosome: | chromosome 7 |
| Location: | 973782 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319350 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATGGGATGGTGTGTATCAAATATGAAGTGAAGTGCCGACGTCCAGAG |
| Internal bar code: | GATTAGCGCGGAAGGAAAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3200 |
| LEAP-Seq percent confirming: | 89.7959 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGGTTGTCCGCTCAC |
| Suggested primer 2: | TCACCCAACACTCGCACTAC |