| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.019718 |
| Chromosome: | chromosome 5 |
| Location: | 1332279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241655 | (1 of 24) IPR001478 - PDZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTCAAAGCAGGCCTCACCACACACACACACACACACACACACACACA |
| Internal bar code: | TTAGTTGGTTTAGAAAGCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3756 |
| LEAP-Seq percent confirming: | 69.0648 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 139 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACTACTGCTGCTCTTGA |
| Suggested primer 2: | TACCGTTTCGTTTCGACCGT |