Insertion junction: CLIP2.019728_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.019728
Chromosome:chromosome 8
Location:831988
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361150 GT90F11,GT90-11 GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGATTGTCCGGCTCTGGCCTGCTGCACACACACACACACACACACACA
Internal bar code:CCCTGATTACCGCAAAGTATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:395
LEAP-Seq percent confirming:23.0769
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTCCCTTGCGCTTTACC
Suggested primer 2:CCAGCACGATTGGCTTTAGC