Insertion junction: CLIP2.019743_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.019743
Chromosome:chromosome 4
Location:437275
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g217919 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTTTTTTACAGCACCGGCTGCTGCATCTACCGTACTACACATCCCGAC
Internal bar code:ATCGGATCCCCGAGATGTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1604
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGGAGGATGATGACGATG
Suggested primer 2:ACGTCGGCACTTCACTTCAT