Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.019761 |
Chromosome: | chromosome 9 |
Location: | 5786297 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g411100 | RPS10 | Cytosolic 80S ribosomal protein S10; (1 of 1) K02947 - small subunit ribosomal protein S10e (RP-S10e, RPS10) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGCCACCCTCGCGGGGGCCACCGAAGCCACCCTGCACGCAGCAGAA |
Internal bar code: | TTCCTGTAATTCGGTTGGTTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4111 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 62 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGCACCGACACACACGTAT |
Suggested primer 2: | AGTACTTGCCAGACTGCACC |