Insertion junction: CLIP2.019801_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.019801
Chromosome:chromosome 3
Location:5931566
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g188850 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCGCACACACAGGCACGGAACGAACTCGGAATGACGGTTTGGCCAAAC
Internal bar code:TTGAGTATGTAAAGGGCGGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1642
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTCGCAATGATCAACTT
Suggested primer 2:ACTGTGGTTAGCAGCCGTAC