Insertion junction: CLIP2.019818_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019818
Chromosome:chromosome 3
Location:7257469
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g207550 ADH8,CAD2 Cinnamyl-alcohol dehydrogenase; (1 of 2) 1.1.1.195 - Cinnamyl-alcohol dehydrogenase / CAD intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACAACTGTCACGTCTTCCCATTTAGGCGCGCCTGATACCCTCTTGCC
Internal bar code:ATCCGTTATGGGGGACCGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3664
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:156
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:156

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTGGAACGTGTTCTCCG
Suggested primer 2:CGTACAAAACAAGGCGTGCA