| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019832 |
| Chromosome: | chromosome 13 |
| Location: | 122544 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562700 | CSB51,TNP34 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTGATTGACAGGTTGACGGTTGGGACAGCTCATGTGCGCTCTCCTCT |
| Internal bar code: | AGTGGAGGCGCTGCTGTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3003 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACAAGGAGGTGGCTGCC |
| Suggested primer 2: | GGGCCGATTCCATATGAGCA |