| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.019838 |
| Chromosome: | chromosome 12 |
| Location: | 5247736 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527700 | CYG19 | (1 of 2) IPR001054//IPR029016//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // GAF domain-like // Nucleotide cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACGCGAGTGCGCGCCGTTCGCAGCTGTTCTGGGGGACACTTGCGGG |
| Internal bar code: | TCCGGTTGTGCCGTTCAGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2372 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATCTACCAGAGTACGGC |
| Suggested primer 2: | ATTGGAGAAGCGTGCAGTCA |