| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.019854 |
| Chromosome: | chromosome 3 |
| Location: | 5953322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189100 | (1 of 1) PTHR31606:SF1 - WBP2 ORTHOLOG, ISOFORM E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAAGTTACCTCGCATACCTGCTGCGGTGAGCCGCCGGTCTTACCAACC |
| Internal bar code: | CATGCTTGGAGAGCGCTCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2118 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCTAACATGTTCCTTCG |
| Suggested primer 2: | GCACACTGACACACACGAAC |