Insertion junction: CLIP2.019882_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.019882
Chromosome:chromosome 6
Location:8433210
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g308300 RRM6 (1 of 2) IPR002877//IPR029063 - Ribosomal RNA methyltransferase FtsJ domain // S-adenosyl-L-methionine-dependent methyltransferase; Putative ribosomal RNA methyltransferase of the RrmJ/FtsJ family CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGTTGTGTGCAATACCGAAGTGGAAGGTAAATTCAAGGTGTGTGCGAT
Internal bar code:GTGCTCTTGGCACAATGGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3997
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCTGGATCACGACATACA
Suggested primer 2:AGGAGGAGGTGAGGGTTGTT