| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019911 |
| Chromosome: | chromosome 3 |
| Location: | 4548461 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176200 | (1 of 1) PTHR22904//PTHR22904:SF364 - TPR REPEAT CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAGGTTGAACAACGCCGTCGCCGCCGCCCGGCGTACCTCCGCATCCA |
| Internal bar code: | AGATTATCACATTAGTTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGTGGCGCATTTTCAAG |
| Suggested primer 2: | TCCACCTGCCTCTACCTACC |