| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019930 |
| Chromosome: | chromosome 7 |
| Location: | 1275492 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322100 | UBQ5 | Ubiquitin fusion protein; (1 of 1) PF13019 - Telomere stability and silencing (Telomere_Sde2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACGCCCTTCCCAACCGCCCCCAACCGAAAACATCCGCGCCCAC |
| Internal bar code: | AGGGTGTCACTCAAAATTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2542 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCCCATGCCATTGATT |
| Suggested primer 2: | AGACGACCAACAACGATGCT |