| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.019936 |
| Chromosome: | chromosome 9 |
| Location: | 5206497 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406851 | CDH1 | Activator and specificity factor for anaphase promoting complex; (1 of 1) K03364 - cell division cycle 20-like protein 1, cofactor of APC complex (CDH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCTATCCCTGCCCCCTGCTGCCCACCTTGACTGCTGCTGTGTGGTC |
| Internal bar code: | GGTTTGCCGGGGTTGTCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACCACTGTCCCTGGCTA |
| Suggested primer 2: | CCATGAACCCTTCCCTTCCC |