| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019951 |
| Chromosome: | chromosome 12 |
| Location: | 4765295 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523100 | (1 of 4) PF05653 - Magnesium transporter NIPA (Mg_trans_NIPA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCTGTGAACGCCATGCTGCTAGCCCCCGCCGCACTCACCAAACGAC |
| Internal bar code: | TTACCTTGTTTGGTGGCAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4757 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCCATAACTTGCCAGCG |
| Suggested primer 2: | TGGTTTCCGAACACCACCAA |