| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019970 |
| Chromosome: | chromosome 12 |
| Location: | 6545860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538150 | (1 of 1) K05019 - chloride channel, nucleotide-sensitive, 1A (CLNS1A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTTGCTGGCAATGGGTTGGGATCCGTGGTTGGGACCCCACCGCTTCG |
| Internal bar code: | TTAGCTTGCAGCGTCGACAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2257 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCTTGCCTGGCTGTCCTC |
| Suggested primer 2: | TGAACATGTCGTCCACTGGG |