Insertion junction: CLIP2.019989_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019989
Chromosome:chromosome 4
Location:2109280
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g219725 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCTCACTCACCCGGTCCCGTGCCTCGCTATCCTGCCTGTCCAGCTGC
Internal bar code:TCGTTGAGAGTTGGATCGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2028
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGGAAGCCTCGTACACG
Suggested primer 2:GTCCTTCGTCCCTAAGCACC