| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019998 |
| Chromosome: | chromosome 7 |
| Location: | 4259734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341650 | (1 of 1) K12848 - U4/U6.U5 tri-snRNP component SNU23 (SNU23) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGGGGGCCAAGGACGGTGACGATGACGACCACGAGGAGACGGCGGC |
| Internal bar code: | ATTTCAAATACATTCTTACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTCCATTCCCCTCCCTC |
| Suggested primer 2: | TTCTGCGCTGCTTTTGTCAC |