Insertion junction: CLIP2.020006_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020006
Chromosome:chromosome 8
Location:537277
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g359550 CDS
Cre08.g359567 (1 of 44) IPR000048 - IQ motif, EF-hand binding site 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTGCTGCTGCTGCTGCTGCTGTAGCCCCGCCGGTGCGGGTGCCAGCA
Internal bar code:GATCATAGTAAACAAGGGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:111
LEAP-Seq percent confirming:5.55556
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCATCACCATCACCACA
Suggested primer 2:CGTCGTACCTGCGATCATGA