Insertion junction: CLIP2.020011_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020011
Chromosome:chromosome 3
Location:276919
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144404 (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAACAAAATTCCGCCATACACGCGCGCGCAGTTACCAACAAATACCGA
Internal bar code:CTGCTTCAGCTCTAGCACTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1408
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAGAGGTGGATCACTCGC
Suggested primer 2:CAGCCAAGTGTTGTCGTGTG