Insertion junction: CLIP2.020012_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020012
Chromosome:chromosome 10
Location:4910084
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g453766 (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTAGCTGCATACCTCAGCGCCTGCACTCCCAGGGGCATGCCCGCTGCG
Internal bar code:TCCGAACCACATGTGTGTGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:7234
LEAP-Seq percent confirming:3.27869
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCATTGTTGGAGCAGCTA
Suggested primer 2:CTCCGTGGTTTCTCCCCTTC