| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020022 |
| Chromosome: | chromosome 13 |
| Location: | 5174382 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607350 | CAH7 | (1 of 2) PTHR11002:SF13 - BETA CARBONIC ANHYDRASE 1; Carbonic anhydrase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCCACCTGCTACAGCTGCTGGCTCCTCACCGCTCATGCCGACATCGA |
| Internal bar code: | GTAATTGTGTAACTCTAAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACCAAGACCCAGCCAG |
| Suggested primer 2: | CAGGCAGGCAGGTTTTAGGA |