Insertion junction: CLIP2.020023_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020023
Chromosome:chromosome 11
Location:4231795
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g481866 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTGCCGGGCGCAAGCACCGGGCGCTGCTGCTGCCCACCCGGCTCGGC
Internal bar code:ATAATAATCAAACACGGTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1840
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTGACTGAAGCAGTGGT
Suggested primer 2:GTGTCAAGTTACCGCCAGGA