Insertion junction: CLIP2.020034_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020034
Chromosome:chromosome 9
Location:2494420
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393050 PRM6,PRM, PRMT6 (1 of 1) PTHR11006//PTHR11006:SF4 - PROTEIN ARGININE N-METHYLTRANSFERASE // PROTEIN ARGININE N-METHYLTRANSFERASE 7; Protein-/Histone-arginine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATGTCGACCCGCTCCCCTAGCACTGTTATAAACCTCACGTGCATGTCT
Internal bar code:AACGGGACTGTACATAACAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:733
LEAP-Seq percent confirming:2.08333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTCCGAATCAGCTACCG
Suggested primer 2:GGTAGTGGAATGGTGGGGTG