| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020043 |
| Chromosome: | chromosome 3 |
| Location: | 2271006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGCGGCAAGGATGTCGTGCCGCAGTCTCTACAGCTGGTTGAAGAGC |
| Internal bar code: | GGAGATCTATAGGATAAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3703 |
| LEAP-Seq percent confirming: | 99.5 |
| LEAP-Seq n confirming: | 199 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 200 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCCCATACTACCCGTGA |
| Suggested primer 2: | GCGCTTTGTTGTGTGTGCTA |