Insertion junction: CLIP2.020053_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020053
Chromosome:chromosome 6
Location:2466943
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269000 (1 of 1) PF09493 - Tryptophan-rich protein (DUF2389) (DUF2389) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGACTTGATTATAGTATATGGCCATGGGTGTGATGCCAGACAGACACG
Internal bar code:TTGTTTGTACGCTACTGTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2310
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCCTTGCAAATCAACCA
Suggested primer 2:TGTCACTGCATCCAAGCACT