Insertion junction: CLIP2.020053_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020053
Chromosome:chromosome 6
Location:2466983
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269000 (1 of 1) PF09493 - Tryptophan-rich protein (DUF2389) (DUF2389) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGCAGATGAATGCTTCCAACCTCAAGGACCGGGACCTGTGGGCGGCG
Internal bar code:TCGAATGTCCTGCCGACCGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1555
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCACTGCATCCAAGCACT
Suggested primer 2:CACGCCTTGCAAATCAACCA