| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.020070 |
| Chromosome: | chromosome 10 |
| Location: | 2120976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433150 | (1 of 1) PF01344//PF13415 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCCTGACGCCTACCAAGGACGCAGCTGGAAGCCCCGGAGCCGGGGGC |
| Internal bar code: | ACTGTAATGTGAGGCAAGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGAGCGTGGGTACTCA |
| Suggested primer 2: | GCATGTCTGGCAATACAGCG |