Insertion junction: CLIP2.020071_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020071
Chromosome:chromosome 10
Location:5113350
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g455231 CSR4 Carbohydrate sulfotransferase-related 4; (1 of 1) 2.8.2.23 - [Heparan sulfate]-glucosamine 3-sulfotransferase 1 / Heparin-glucosamine 3-O-sulfotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACATACCCACCCACACCACGCCCACCCCGGACACACCAACTCCCCAC
Internal bar code:CAGCAAGCTCTAGAGATTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2251
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCCAGGAGACAGGAGAGG
Suggested primer 2:ATCACCCTGGAAGAAGTGCG