Insertion junction: CLIP2.020102_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020102
Chromosome:chromosome 2
Location:5718836
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g116450 RPE2 (1 of 2) K01783 - ribulose-phosphate 3-epimerase (rpe, RPE); Ribulose phosphate-3-epimerase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTGATGTGAAGTTGTTAAGCCAGGGGGGAAAGCGTGTGGACGCAGGC
Internal bar code:AGGTTCTTTGACTCTTTAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2519
LEAP-Seq percent confirming:85.9649
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACCTGGATGTTGAGGTC
Suggested primer 2:TAGTGAGTCCGCTTTGCCTG