Insertion junction: CLIP2.020103_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020103
Chromosome:chromosome 6
Location:2284460
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267250 MKP2 (1 of 3) K04459 - dual specificity MAP kinase phosphatase (DUSP, MKP); Dual-specificity protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGCCGATGCGCCCCACACGGCCGCCTGGTCGATGCTCCAAACACCGCT
Internal bar code:TGGAGACGGATGTTGAGTTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3772
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCTGTTTACCATTCCGCC
Suggested primer 2:GACACTCTGCCACTGAAGCT