Insertion junction: CLIP2.020115_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020115
Chromosome:chromosome 6
Location:5818605
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g289150 MTP5 Metal Transport Protein 5; (1 of 1) K14696 - solute carrier family 30 (zinc transporter), member 9 (SLC30A9, ZNT9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCATTTGACGAGGACAGCGGCACGCCCGCCCCCACGCTCGCACCCGC
Internal bar code:CACGCACTGATTGTAACCCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2000
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGATTCTTCCTGCACCACCC
Suggested primer 2:GCAGCCGTTGTCGAAGTTTT