Insertion junction: CLIP2.020133_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020133
Chromosome:chromosome 3
Location:8182940
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g200400 (1 of 1) PTHR30383 - PERIPLASMIC BROAD-SPECIFICITY ESTERASE/LIPASE/PROTEASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCAGACGGGAGTTCGGTAGCAGTAATACCGCAACACAGCTCGCGAAA
Internal bar code:TTCGGTTCGGGTGAGCGGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2518
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCTACCCGCATCACTC
Suggested primer 2:TCAGTCGTCTAGGTCCAGCA