| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020144 |
| Chromosome: | chromosome 16 |
| Location: | 516427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692751 | PDI3 | Protein disulfide isomerase 3; (1 of 1) K13984 - thioredoxin domain-containing protein 5 (TXNDC5, ERP46) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTCGGAACCCGAGTCCAGCGAATACGGATGCTGCACCGACCCCAGA |
| Internal bar code: | GGTCGTGGTGCTTTGAAATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2150 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGCAGCTGCAACTCAAA |
| Suggested primer 2: | CAGGCGTCTCTGGAGTGTTT |