Insertion junction: CLIP2.020165_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020165
Chromosome:chromosome 8
Location:657284
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360300 SDR11 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.300//1.1.1.330 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) // Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGTGTGCTTGAGACTTCCAAGGGTGAGGGCCGCGGGCTCTTGACCAG
Internal bar code:GTCAGTTTGTAGACGTCGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1577
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCCTCTTTTCCACCACC
Suggested primer 2:CGAGGGTTGTGCGTCTAGTT