Insertion junction: CLIP2.020165_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020165
Chromosome:chromosome 8
Location:657290
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360300 SDR11 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.300//1.1.1.330 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) // Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAACCCACCCGCGTTGTTGACGAGCACATCGATGCGCCCGCGGCCAG
Internal bar code:ACACTCTATGTGGCTCACTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3327
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGGTTGTGCGTCTAGTT
Suggested primer 2:CCTCCCTCTTTTCCACCACC