| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020173 |
| Chromosome: | chromosome 6 |
| Location: | 4029480 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278215 | HIBCH1 | (1 of 1) 3.1.2.4 - 3-hydroxyisobutyryl-CoA hydrolase; 3-hydroxyisobutyryl-CoA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGCCGTCCTTACCTCCGCATCGCTGCCAATGTGGACTTCAGAAAAGC |
| Internal bar code: | ACACACTCATGGAAAGTCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3096 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTTCCGGTTGCACTTGG |
| Suggested primer 2: | ACGGTTGTACACAGCGACAT |