Insertion junction: CLIP2.020176_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.020176
Chromosome:chromosome 2
Location:4928491
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g110100 MLO1 (1 of 1) PTHR10891//PTHR31942//PTHR31942:SF1 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // FAMILY NOT NAMED // MLO-LIKE PROTEIN 1; MLO family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGCGCTGACGGTGGTACGTCGCGCTTCGGCCTGTCCGCAAACACAGG
Internal bar code:TACGAGAATGGGATTCGCGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1426
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTACATGCCTATGCCCAT
Suggested primer 2:TGCTGCGCAACTTCAACTTC